LAG3/IL2-Luc/Jurkat
CBP74079
| I. Background | |
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淋巴細(xì)胞活化基因 3 (LAG-3)，又名 CD223，是一種 I 型跨膜蛋白，屬于免疫球蛋白超家族 (IgSF)。LAG-3 蛋白與 CD4 有同源結(jié)構(gòu)，兩者的胞外區(qū)均具有四個 Ig 樣結(jié)構(gòu)域，但 LAG-3 在膜遠(yuǎn)端 Ig 樣結(jié)構(gòu)域內(nèi)還含有一種額外的環(huán)狀結(jié)構(gòu)，使其與MHC II的親和力較CD4高100倍。LAG-3 在活化的 T 細(xì)胞、B 細(xì)胞、漿細(xì)胞樣樹突狀細(xì)胞 (DC) 和 NK 細(xì)胞上表達(dá)。LAG3 可結(jié)合 MHC II類 (MHC II)分子外，也可結(jié)合纖維蛋白原樣蛋白 1（FGL-1）、α-突觸核蛋白原纖維 (α-syn) 和凝集素半乳糖凝集素-3 (Gal-3) 以及淋巴結(jié)竇內(nèi)皮細(xì)胞 C 型凝集素 (LSECtin) 。 |
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| II. Description | |
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LAG3 IL2-Luc Jurkat 報告基因藥靶模型很好的模擬了體內(nèi) LAG3 的信號轉(zhuǎn)導(dǎo)過程，原理見下圖所示。
Figure 1. LAG3 IL2-Luc Jurkat 細(xì)胞模型原理圖
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| III. Introduction | |
| Host Cell: | Jurkat |
| Expressed gene: |
LAG3-IL2-Luc |
| Stability: | 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) |
| Synonym(s): |
CD223, LAG-3, Ly66, lymphocyte activating 3, lymphocyte-activation gene 3, LAG3, Lag3, IL-2 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter |
| Freeze Medium: | 90% FBS+10% DMSO |
| Culture Medium: |
RPMI-1640+10%FBS+1 μg/ml Puromycin+10 μg/ml Blasticidin
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| Mycoplasma Testing: | Negative |
| Storage: | Liquid nitrogen |
| Application(s): | Functional(Report Gene) Assay |
| IV. Description of Host Cell Line | |
| Organism: | Human |
| Tissue: | Peripheral blood |
| Disease: | Childhood T acute lymphoblastic leukemia |
| Morphology: | Lymphoblast |
| Growth Properties: | Suspension |
| V. Representative Data | |
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Figure 2. Recombinant Jurkat stably expressing human LAG3. |
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Figure 3. Detect Luciferase assay by Ultra Luciferase Detection Kit CBPH0001（we strongly suggest to purchase from Cobioer). Dose Response of Anti-LAG3 Blocking Antibody in LAG3 IL2-Luc Jurkat (C10) With Raji.
